Skip Navigation


Carcinogenesis Advance Access originally published online on April 1, 2004
Carcinogenesis 2004 25(9):1559-1565; doi:10.1093/carcin/bgh158
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
25/9/1559    most recent
bgh158v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Gauthier, N.
Right arrow Articles by Jeannin, J.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gauthier, N.
Right arrow Articles by Jeannin, J.-F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis vol.25 no.9 © Oxford University Press 2004; all rights reserved.

ARTICLE

Tumour-derived and host-derived nitric oxide differentially regulate breast carcinoma metastasis to the lungs

Nolwenn Gauthier1, Sylvia Lohm2, Claude Touzery3, Aurélie Chantôme1, Bernard Perette3, Sylvie Reveneau1, François Brunotte3, Lucienne Juillerat-Jeanneret2 and Jean-François Jeannin1,4

1 EPHE/INSERM/Laboratoire d'immunologie et immunothérapie des cancers, Unité 517 et IFR 100, Université de Bourgogne, Dijon, France, 2 Institut Universitaire de Pathologie, CHUV, Lausanne, Switzerland and 3 Service de médecine nucléaire, Centre Georges-François Leclerc, et IFR 100 de l'INSERM, Dijon, France

4 To whom correspondence should be addressed Email: jean-francois.jeannin{at}u-bourgogne.fr

To study the role of nitric oxide (NO) in lung metastasis of breast carcinoma, we isolated two cell clones (H and J) from the parental EMT-6 murine breast carcinoma cell line, based on their differential NO production. In vitro, EMT-6 J cells, but not EMT-6H cells, constitutively expressed inducible NO synthase (NOS II) and secreted high levels of NO. IL-1ß increased NO production in both clones, and TNF-{alpha} had a synergistic effect on IL-1ß-induced NO production, but NO production by EMT-6 J cells was always higher than by EMT-6H cells. Proliferation, survival and adhesion to lung-derived endothelial cells of both clones were similar and were not affected by NO. In vivo, both clones similarly located in the lungs of syngeneic mice 48 h after injection. However, EMT-6H cells were significantly more tumorigenic than EMT-6 J cells as assessed at later time points. Injection of EMT-6 J cells and simultaneous treatment of mice with aminoguanidine (AG), a NOS II inhibitor, significantly increased tumour formation. Injection of EMT-6H and EMT-6 J cells into NOS II-deficient mice resulted in a significant survival increase as compared with wild-type animals. Simultaneous administration of AG increased the death rate of NOS II-deficient mice injected with EMT-6 J cells. These results demonstrate that: (i) NO does not influence the early stages of tumour metastasis to the lungs and (ii) NOS II expression in tumour cells reduces, while NOS II expression in host cells enhances, tumour nodule development. In conclusion, the cellular origin and the local NO production are critical in the metastatic process.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
J.-H. Chen, H.-H. Lin, T.-A. Chiang, J.-D. Hsu, H.-H. Ho, Y.-C. Lee, and C.-J. Wang
Gaseous Nitrogen Oxide Promotes Human Lung Cancer Cell Line A549 Migration, Invasion, and Metastasis via iNOS-Mediated MMP-2 Production
Toxicol. Sci., December 1, 2008; 106(2): 364 - 375.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Gautier, A. Klein, V. Deckert, C. Desrumaux, N. Ogier, A.-L. Sberna, C. Paul, N. Le Guern, A. Athias, T. Montange, et al.
Effect of Plasma Phospholipid Transfer Protein Deficiency on Lethal Endotoxemia in Mice
J. Biol. Chem., July 4, 2008; 283(27): 18702 - 18710.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Stempelj, M. Kedinger, L. Augenlicht, and L. Klampfer
Essential Role of the JAK/STAT1 Signaling Pathway in the Expression of Inducible Nitric-oxide Synthase in Intestinal Epithelial Cells and Its Regulation by Butyrate
J. Biol. Chem., March 30, 2007; 282(13): 9797 - 9804.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. F. Weber, F. C. Gaertner, W. Erl, K.-P. Janssen, B. Blechert, B. Holzmann, H. Weighardt, and M. Essler
IL-22-Mediated Tumor Growth Reduction Correlates with Inhibition of ERK1/2 and AKT Phosphorylation and Induction of Cell Cycle Arrest in the G2-M Phase
J. Immunol., December 1, 2006; 177(11): 8266 - 8272.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
A.-M. Simeone, S. Colella, R. Krahe, M. M. Johnson, E. Mora, and A. M. Tari
N-(4-Hydroxyphenyl)retinamide and nitric oxide pro-drugs exhibit apoptotic and anti-invasive effects against bone metastatic breast cancer cells
Carcinogenesis, March 1, 2006; 27(3): 568 - 577.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C. Khanna and K. Hunter
Modeling metastasis in vivo
Carcinogenesis, March 1, 2005; 26(3): 513 - 523.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.