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© 1995 Oxford University Press

research-article

Frequent T:A->G:C transversions in X-irradiated mouse cells

Jianling Yuan, Toni M. Yeasky, Monica C. Rhee and Peter M. Glazer 1

Department of Therapeutic Radiology, Yale University School of Medicine 333 Cedar Street, New Haven, CT 06510, USA

1To whom correspondence should be addressed

Ionizing radiation is a known carcinogen and teratogen. However, the point mutations produced by Ionizing radi ation in mammalian cells have not been fully characterized. Determination of a characteristic spectrum of X-ray Induced mutations in mammalian cells could provide clues to cellular repair processes and could serve as a marker of individual exposure to radiation. Mouse fibroblasts containing in their genome multiple copies of a recoverable {lambda} phage shuttle vector were used to detect and analyze radiation-induced point mutations in the supF mutation reporter gene. Following fractionated doses of ionizing radiation, a unique mutational spectrum notable for a high proportion of T:A->G:C transversions (57%) was found. This pattern was distinct from the spectra of UV-induced and spontaneous mutations detected in the same mouse cell assay system (mainly C:G->T:A transitions). The pre dominance of T:A->G:C transversions and the pattern of mutation hot-spots are consistent with a possible role for polymerase ß in the repair of X-ray-damaged DNA. These results may also help to define a distinctive mutational signature of X-ray exposure in mammalian cells.


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